Application

Anti-Connexin 32 Antibody, clone M12.13 detects level of Connexin 32 & has been published & validated for use in WB, IH.

Immunohistochemistry: acetone or unfixed tissues recommended. Epitope is internal, paraffin sections are untested.

Works with lightly formaldehyde-fixed tissue.

Western blot: Detects a 27 kDa Connexin 32 polypeptide in blots of rat hepatocyte gap junctions and an additional 47 kDa band corresponding to aggregates.

Optimal working dilutions must be determined by end user.

Research CategoryCell Structure

Research Sub CategoryAdhesion (CAMs)

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Liquid in 0.02M PB, 0.25M NaCl, containing 0.1% sodium azide.

Format: Purified

Specificity

Monoclonal antibody MAB3069 was produced by the immunization of BALB/c mice with isolated rat junctional complexes, prepared by the method of Stevenson and Goodenough (1988). Epitope mapping, hydropathy plotting, and topological studies suggest that this antibody is very likely directed against an epitope located between residues 95-125 in the central cytoplasmic loop of rat Cx32.

Storage and Stability

Maintain at 2-8°C in convenient undiluted aliquots for up to 6 months.